Environmental Engineering Reference
In-Depth Information
taBle 4.1
cellulase, hemicellulase, and cell Wall modifying enzymes expressed in Plants
expression
strategy/
targeting
activity assay/
temperature and ph
optima
enzyme/
source
native or
modified
host/tissue/
Phenotype
Promoter type/
source
maximum
yield
references
endoglucanases
E1 endoglucanase/
Acidothermus cellulolyticus
Native
Tobacco/Leaves/
Normal
Stable nuclear/
Chloroplasts
Light responsive/
Tomato RbcS-3C
1.35% TSP
MUCase/81°C and
pH 5.25
Dai et al.
2000a
E1 endoglucanase/
Acidothermus cellulolyticus
Native
Potato/Leaves/
Normal
Stable nuclear/
Chloroplasts
Light responsive/
Tomato RbcS-3C
2.6% TSP
MUCase/
ND
Dai et al.
2000b
E1 endoglucanase CD/
Acidothermus cellulolyticus
Native
Arabidopsis thaliana /
Leaves/Normal
Stable nuclear/
Apoplast
Constitutive/
CaMV 35S
26% TSP
MUCase/
ND
Ziegler et al.
2000
E1 endoglucanase/
Acidothermus cellulolyticus
Native
Tobacco/Leaves/
Normal
Stable nuclear/
Chloroplasts
Constitutive/
CaMV 35S
ND
MUCase
ND
Jin et al.
2003
E1 endoglucanase/
Acidothermus cellulolyticus
Native
Tobacco/Leaves
Normal
Normal
Normal
Stable nuclear/
Apoplast
Chloroplast
Cytosol
Constitutive/
CaMV 35S
0.33% TSP
0.0007% TSP
0.0006% TSP
MUCase/
ND
Ziegelhoffer
et al. 2001
E1 endoglucanase CD/
Acidothermus cellulolyticus
Native
Tobacco/Leaves
Normal
Normal
Normal
Stable nuclear/
Apoplast
Chloroplast
Cytosol
0.4% TSP
0.074% TSP
0.0046% TSP
E1 endoglucanase/
Acidothermus cellulolyticus
Native
Tobacco/Leaves
Stable nuclear/
Apoplast
Constitutive/
CaMV35S
0.25% TSP
MUCase
Dai et al.
2005
E1 endoglucanase CD/
Acidothermus cellulolyticus
Native
Rice/Leaves
Normal
Stable nuclear/
Apoplast
Constitutive/
CaMV35S
4.9% TSP
MUCase
AFEX-treated corn stover
AFEX-treated rice straw
CMC
Avicel
Oraby et al.
2007
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