Environmental Engineering Reference
In-Depth Information
taBle 23.2
Internet resources available for Brachypodium research
resource
Institution
url
Purpose
Brachypodium.org
Oregon State
University
http://www.
brachypodium.org/
Overview and links to
Brachypodium research and
resources
Brachybase
Oregon State
University
http://www.brachybase.
org/
Genome browser, EST alignments,
Illumina-based RNAseq data, and
empirical TAU gene model
predictions
Brachypodium
genome browser
Munich Information
Center for Protein
Sequence (MIPS)
http://mips.helmholtz-
muenchen.de/plant/
brachypodium/index.jsp
Genome browser, BLAST server
Phytozome
JGI and Center for
Integrative Genomics
http://www.phytozome.
net/
Comparative genomic studies of
green plants
CoGe
University of
California, Berkeley
http://synteny.cnr.
berkeley.edu/CoGe/
Comparative genomic studies
Brachypodium
Genome Resources
USDA-ARS Western
Regional Research
Center
http://brachypodium.
pw.usda.gov/
Protocols, publications, SSR
markers, germplasm, T-DNA
insertional mutant lines, maps,
and other resources
Garvin Laboratory
USDA-ARS Plant
Science Research
Unit
http://www.ars.usda.gov/
pandp/docs htm?
docid=18531
Brachypodium populations and
crossing protocol
Modelcrop
JGI
http://www.modelcrop.
org/index.html
Genome browser, BLAST server,
physical map, and comparative
maps
Physical map
University of
California, Davis
http://phymap.ucdavis.
edu:8080/
brachypodium/
Physical map
BrachyTAG
John Innes Centre
http://www.brachytag.
org/
T-DNA insertional mutant lines
USDA National Plant
Germplasm System
USDA
www.ars-grin.gov/npgs/
Germplasm
GrainGenes
USDA-ARS Western
Regional Research
Center
http://wheat.pw.usda.
gov/GG2/index.shtml
Comparative genomics, cMAP
viewer
on the fact that immature anthers will dehisce shortly after removal from the plant. In brief, florets
with feathery stigmas and anthers that have not yet dehisced are suitable for crossing. One floret is
emasculated by peeling back the lemma and removing the anthers. The anthers that will serve as
pollen donors are dissected from florets and permitted to swell for 10-30 min before they release
fresh pollen for crossing. Pollen or entire anthers are then introduced to the emasculated floret, and
the lemma is closed to prevent desiccation and protect the developing seed. With practice, crosses
can be made in 5-10 min or less.
23.4 GenomIc resources
An extensive infrastructure of genomic resources has been (and continues to be) assembled for
Brachypodium, including cDNA libraries, BAC libraries, a large expressed sequence tag (EST)
