Environmental Engineering Reference
In-Depth Information
(a)
(b)
(c)
(d)
(e)
(f )
(g)
(h)
(i)
FIGure 23.1 (see color insert)
Adult plant comparisons and transformation. Flowering plants of maize (a),
switchgrass (b), and Brachypodium (c) compared with the same 32-cm ruler, indicated with white arrows. The
small size of Brachypodium is an advantage for a model system. (d-f) Comparison of
B. distachyon
and
B. syl-
vaticum
. (e) The annual species
B. distachyon
(left) next to its perennial relative
B. sylvaticum
(right). Bar is 15
cm. Inflorescences of
B. distachyon
(d) and
B. sylvaticum
(f). Note the exerted anthers of the outcrossing
B. syl-
vaticum
and the enclosed anthers of the inbreeding
B. distachyon
. (g) Embryogenic Brachypodium callus. The
yellow structured regions are competent to regenerate plants and are used for transformation. Bar is 0.5 cm. (h)
Plants regenerating from transgenic callus. After transformation and selection, a mixture of dying (brown) and
healthy (yellow) callus can be seen. When placed in the light, healthy callus will turn green and regenerate plant-
lets. Plate is 10 cm in diameter. (i) Close-up of region in regeneration plate designated by the arrow in panel h.
