Chemistry Reference
In-Depth Information
Peptide Synthesis
a.
N-protection:
O
O
R
1
base
CO
2
-
(CH
3
)
3
CO
C
O
C
OC(CH
3
)
3
+N
H
3
C
di-
t
-butyl dicarbonate
H
O
R
1
CO
2
-
(CH
3
)
3
CO
C
NH
C
H
BOC or
P
b.
Polymer attachment:
R
-Cl
-
1
-
P
NH
C
CO
2
+
Cl
CH
2
H
R
O
1
P
NH
C
C
O
CH
2
H
= polymer
c.
Deprotection (removal of the protecting group):
O
R
O
1
HCl
(CH
3
)
3
CO
C
NH
C
C
O
CH
2
CH
3
CO
2
H
H
R
O
1
H
2
N
C
C
O
CH
2
+CO
2
+(CH
3
)
2
CCH
2
H
