CALLUS CULTURE TECHNOLOGY OF SPRING SOFT WHEAT STRESS TOLERANT VARIETIES SELECTION - Biochemistry and Biotechnology: Research and Development - page 60

All these data confirm the tolerance of regenerant to high H + concentration. Also it is
important that regenerant surpass the parental variety under control conditions. The major
part of ploughed field has neutral soil conditions. So the regenerant superiority in these
conditions can be the determinant factor in selection material choosing.
Regenerants of Taezhnaja created on control medium also showed high level of tolerance
in comparison with their parental variety (Table 6).
C ONCLUSION
Spring soft wheat varieties in vitro selection for drought, salinity and acidity tolerance
technology was developed at the base of the local Siberian breeding material. Medium
hormone composition and selective agent levels were optimized. Explants characteristics
were established and most sensitive to callus culture conditions genotypes were chosen.
Some mechanisms of spring soft wheat callus culture development were detected.
Multiple regeneration phenomenon of wheat was investigated for the first time. The
correlation of this phenomenon with donor plant and regenerant tilling capacity and their
vegetation period was detected.
Method of cereal crops test for acidity tolerance was improved. High tolerance to soil
salinity and acidity of regenerants created on selective media was showed in physiological
tests. So the effectiveness of developed in vitro selection technology was confirmed.
Three regenerants were transfered to prior varieties test department according to the
research data of two-year field experiments.
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