CALLUS CULTURE TECHNOLOGY OF SPRING SOFT WHEAT STRESS TOLERANT VARIETIES SELECTION - Biochemistry and Biotechnology: Research and Development - page 58

P HYSIOLOGICAL T OLERANCE T ESTS OF R EGENERANT L INES
C REATED ON S ELECTIVE M EDIA
Seeds of 26 regenerant lines created on selective media and of 20 lines created on
nonselective media were obtained. The third generation of regenerants was tested for salinity
(NaCl 1,68 %) or acidity (pH 3,0) tolerance according to the selection direction.
C OMPARATIVE C HARACTERIZATION OF S ALINITY T OLERANCE
Regenerants of KS-1607 and Minusa varieties created on medium containing salt (NaCl
0,42%) were more tolerant under stress conditions according to the test data (Table 5).
Figure 5. Influence of NaCl (1,68%) on plantlets growth of KS-1607 and it`s regenerants, created on
control medium (RC).
The regenerants created on control medium also showed some tolerance - decrease of
their growth activity in salt solution in comparison with data in clear water was lower than
parental genotype KS-1607 one`s (Figure 5). This feature of RC regenerants results from
presence of selection for cells with high regeneration activity under callus culture conditions.
Difference in reaction of samples (regenerant lines) can be explained by intragenotype
polymorphism of parental cultivar as well as by somaclonal variations that are the base of all
in vitro selection of stress-tolerant varieties technologies [[16], [1], [17]] . It seems that we can
talk about inherited features created under callus culture conditions because they are showed
in the tests conducted on third generation.
A CIDITY T OLERANCE C HARACTERIZATION
Growth stimulation of plantlets was detected when roll test of acid tolerance was used.
Filter paper and wheat seeds increase medium pH up to not stress level during
germination.