CALLUS CULTURE TECHNOLOGY OF SPRING SOFT WHEAT STRESS TOLERANT VARIETIES SELECTION - Biochemistry and Biotechnology: Research and Development - page 56

Table 6. К red of acid-tolerant regenerants (RA), regenerants created on control medium
(RC) and parental genotypes

RA
(Minusa)

RC (Taezgnaja)
1

RC (Taezhnaja)
2

Parametrs Minusa Taezhnaja

К l 0,47 0,60 0,18 0,29 0,31

K wp 0,52 0,71 0,20 0,36 0,36

К i

K wr 0,32 0,51 0,09 0,19 0,23

K ws 0,68 0,80 0,26 0,45 0,41

К red 0,51 0,67 0,18 0,32 0,33

Two PEG concentration influence on wheat callus culture growth and development were
investigated (Table 4). The level of PEG of 22% induced stable decreasing of regeneration
capacity. However, leafs formed under that conditions looked glassy and were easy affected
by fungal diseases. The lower PEG concentration didn`t induced any changing of culture
growth parameters in comparison with control condition s. The level of PEG 18% was
considered suitable for selection in vitro as a result of our research and according to research
data of other scientists [[13]] .

M ULTIPLE R EGENERATION P HENOMENON

Regenerant yield is one of the main characteristic of callus culture methods applying
effectiveness. Regenerants yield can be also increased due to multiple regeneration
phenomenon (MRP) (Figure 2). The higher frequency of MRP at late cultivars callus culture
in comparison with early ones was showed for barley [[14]] . There is no similar data for
wheat. Frequency of MRP in early wheat cultivar`s (Taezhnaja, Novosibirskaja 15) callus
culture was twice as higher as in late cultivar`s (Minusa, KS-1607) one, 4,1±0,81 and
1,56±0,27%, respectively. It seems that separate regeneration sites can more often organized
in several centre of attraction because of early genotypes original high development rate.

Figure 2. Callus with MRP.