CALLUS CULTURE TECHNOLOGY OF SPRING SOFT WHEAT STRESS TOLERANT VARIETIES SELECTION - Biochemistry and Biotechnology: Research and Development - page 51

Chapter 8
C ALLUS C ULTURE T ECHNOLOGY OF S PRING S OFT
W HEAT S TRESS T OLERANT V ARIETIES S ELECTION
V. Ju. Stupko * and N. V. Zobova
Krasnoyarsk research institute of an agriculture of Russian Academy of Agriculture,
Krasnoyarsk, Russia
A BSTRACT
Spring soft wheat varieties in vitro selection for drought, salinity and acidity
tolerance technology for Siberian local breeding material was developed. Multiple
regeneration phenomenon of wheat callus culture was investigated for the first time.
Method of cereal crops test of acidity tolerance was improved.
Keywords : Triticum aestivum, in vitro, drought, salinity, low pH, tolerance, test methods
I NTRODUCTION
Earth population is growing and increase of food supply is demanded. New territories
which development is impossible without new high-adaptive crop varieties creation are
involved in crop rotation. Soil salinity, acidity and drought become the factors which limit
crop yield. Classic methods of selection often can`t solve this problem in a required short
space of time [[1]] . Genetically modified plants are forbidden to be grown in most part of
developed countries in addition.
So far callus culture in vitro can become effective ecology safe technology of high stress
tolerant wheat varieties creation. This method are widely used in crop selection [[2]] ,
especially wheat one [[3], [3], [5]] . Technology of spring soft wheat varieties selection for
drought, soil acidity and salinity tolerance at the base of local Siberian breading material was
developed.