Biomedical Engineering Reference
In-Depth Information
Figure 3. Colony morphology of
Lactococcus
lactis
ssp.
lactis
strain 194.
Table. Viability patterns for different strains of Lactococcus lactis
Lactococcus lacti
s
MGU
Lactococcus lactis
194
Lactococcus lacti
s
729
Period of
starvation
Viable cells/ml
with Live/Dead
Viable cells/ml
with Live/Dead
Viable cells/ml
with Live/Dead
CFU/ml
CFU/ml
CFU/ml
0 weeks. (1,33ﾱ0,14)х10
7
(1,05ﾱ0,12)х10
7
(4,33ﾱ0,47)х10
7
(1,52ﾱ0,17)х10
7
(2,1ﾱ0,23)х10
7
(1,25ﾱ0,13)х10
7
2 weeks. (2,78ﾱ0,3)х10
4
(3,40ﾱ0,37)х10
7
(5,50ﾱ0,61)х10
4
(3,45ﾱ0,38)х10
7
(3,00ﾱ0,33)х10
4
(3,29ﾱ0,36)х10
7
5 weeks. (5,6ﾱ0,61)х10
3
(3,23ﾱ0,35)х10
7
(3,87ﾱ0,4)х10
4
(3,22ﾱ0,35)х10
7
(1,85ﾱ0,2)х10
4
(3,69ﾱ0,41)х10
7
6 weeks.
(5,20ﾱ0,57)х10
3
(4,59ﾱ0,5)х10
7
(1,8ﾱ0,2)х10
4
(4,47ﾱ0,49)х10
7
(7,4ﾱ0,81)х10
4
(6,79ﾱ0,74)х10
7
C
ONCLUSION
We studied viability patterns of 3 strains of
Lactococcus lactis
using different methods.
Formation of VBNC cells of lactococci was registered after 2 weeks when cells were
incubated in carbohydrate starvation conditions.
Ability to form VBNC cells and speed of culturability loss are strain dependent.
Obtained data can be used to assess numbers of viable lacococcus cells in environmental
and food samples and in biomedical preparations.
R
EFERENCES
[1]
B. Ganesan, R. Mark, M.R. Stuart, B.C. Weimer:
Appl. Environ. Microbiol
., 73 (8),
2498 (2007).
[2]
J.J. Byrd, H.-S. Xu, R.R. Colwell:
Appl. Environ. Microbiol
., 57(3), 875 (1991).
[3]
M. Eschbach, K. Schreiber, K. Trunk, J. Buer, D. Jahn, M. Schobert:
J. Bacteriol.,
186
(14), 4596 (2004).
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