DEVELOPMENT OF HRP-FUNCTIONALIZED CARBON-COATED IRON NANOPARTICLES USING ARENEDIAZONIUM TOSYLATES - Biochemistry and Biotechnology: Research and Development - page 42

appeared in the range 1700-1600 cm -1 (amide I), caused by C=O stretching vibrations of
peptide linkages, and 1600-1500 cm -1 (amide II), resulted from a combination of N-H in
plane bending and C-N stretching vibrations of the peptide groups. The broad adsorption
band at 550 cm -1 belongs to Fe@C nanoparticles [4]. After ADT-modification in ATR-IR
spectra of nanoparticles the characteristic adsorption bands of benzene ring vibration in range
500-1100 cm -1 and COOH group vibration at 1346 cm -1 are appeared [4, 11].
Figure 3. DAP (2,3-diaminophenazine) formation in the presence of H 2 O 2 as a function of time. The
reaction is catalyzed by free HRP (red line 1) and HRP immobilized onto carbon-coated iron
nanoparticles (blue line 2).
Figure 4. Enzymatic activity of free (control) HRP (red column) and HRP immobilized onto carbon-
coated iron nanoparticlesafter preparation (blue column) and after storing 1 and 7 days.