Biomedical Engineering Reference
In-Depth Information
Chapter 6
D
EVELOPMENT OF
HRP-F
UNCTIONALIZED
C
ARBON
-C
OATED
I
RON
N
ANOPARTICLES
U
SING
A
RENEDIAZONIUM
T
OSYLATES
Alexandra G. Pershina,
1,
*
Olga A. Lytkina,
1
Pavel S. Postnikov,
2
Marina E. Trusova,
2
Dmitri Yu. Svitich,
2
Viktor D. Filimonov
2
and Alexey E. Sazonov
1
1
Siberian State Medical University, Tomsk, Russian Federation
2
Tomsk Polytechnic University, Tomsk, Russian Federation
A
BSTRACT
The present article describes the method of immobilization of biomolecules
(Horseradish peroxidase) on carbon-coated iron nanoparticles using arenediazonium
tosylates. As a result, a magnetically controlled complex of biomolecules and
nanoparticles was created. The complex formation was confirmed by FT-IR
spectroscopy. It has been shown that peroxidase attached to nanoparticles preserves its
enzymatic activity.
Keywords:
Carbon-coated nanoparticles, diazonium salt, surface modification, HRP,
immobilization, enzymatic activity
I
NTRODUCTION
The constructions based on superparamagnetic nanoparticles, which are covalently bound
with protein molecules (such as enzymes, antigens, antibodies), are particularly useful in
biomedical diagnostics and environmental monitoring [1]. Due to their small size and high
surface area, these constructions effectively interact with the target molecule directly as well
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