Biomedical Engineering Reference
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2.
It has been shown during cultivation in flasks that accumulation of TBI protein in TB
plus medium did not depend on the time of addition of nalidixic acid at an optical
density of 0.8, 1.2 and 1.5 and constituted, on an average, 16.5 ± 0.4 %.
3.
When cultured in a laboratory fermenter the maximal content of target protein in
biomass reached 18.2 % following induction at an optical density of 0.8.
4.
Not only quantitative, but also qualitative characteristics of target TBI protein were
improved as a result of optimization of the process of cultivation of recombinant,
E.coli
JM 103/pTBI strain.
A
CKNOWLEDGMENTS
We are grateful to T.I. Rozhkova and E.Y. Roslyakova for technical assistance in
performing pirogenecity and LAL tests. We thank E.G. Kharitonova for translating the paper.
R
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