PRODUCTION OF NITRIC OXIDE BY ENDOTHELIAL CELLS INFECTED WITH HERPES SIMPLEX VIRUS TYPE 1, UNDER THE INFLUENCE OF INTERFERON - Biochemistry and Biotechnology: Research and Development - page 23

decrease in production of NO, at higher concentrations its action leads to a gradual increase in
the synthesis of NO [18]. Infection of endothelial cells with HSV-1 at MOI 0.1 led to rapid
activation of synthesis of NO, whereas at lower MOI nitric oxide production was decreased.
Under the joint action of IFN-α (pretreatment) and HSV-1 was observed suppression of NO
production by ECs to 24 hours, which coincided with inhibition of virus reproduction.
Activation of nitric oxide synthesis to 48 hours coincided with both an increase in virus
reproduction in this series of experiments, and with the activating effect of IFN-α in the
absence of viral infection found in the first series of experiments.
Study was supported partly by Grant ISTC#3505.

Figure 4. The EPR spectra of a sample of the culture medium of human vascular endothelial cells after
cultivation with interferon-α for 24 h. After incubation, before preparing of the samples in medium
were added ascorbate (10 -5 M) and hemoglobin (5x10 -6 M). Conditions of registration of EPR spectra:
microwave power 20 mW, magnetic field modulation amplitude 4 G, the temperature measurement of
77 K.

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