Biomedical Engineering Reference
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Consequently, already after 3 hour contacts IFN - α (1х106 IU/ml) with ECs there was
activation of oxide nitrogen production. Earlier it has been shown that activation of
endothelial iNOS occurs earlier, than for other types of cells [15-16]. It has been established
that levels мRNA for endothelial iNOS were maximum in 2 hours and further gradually
decreased even at constant stimulation. At the same time it is known that macrophages мRNA
for iNOS it is found out not earlier than in 4-6 hours and further synthesis NO only increases
till 2-3 days in the presence of a substratum [1, 13]. This fast increase and the subsequent
delay of synthesis iNOS in endothelial cells can reflect a functional difference between two
types of cells - endothelial and macrophages making NО after stimulating agents effect.
a
1,1
1,0
1
0,9
2
0,8
4
3
0,7
0
10
20
30
40
50
Time after infection, hrs
Figure 2. Production of oxide nitrogen (a) and a virus reproduction (b) in human vascular endothelial
cells after infection with HSV-1 at MOI (TCID
50
/ml): 1 - 0,1; 2 - 0,01; 3 - 0,001 and 4 - 0,0001.
For the investigation of HSV-1 influence on ECs ability to synthesize nitric oxide
monolayer cultures were infected with a virus with MOI 0,1-0,0001 TCID/
50
. Selected
samples of cultural medium of infected and control cells were tested. In samples the nitrites
levels and an infectious virus were tested in dynamics. Results of experiments are presented
on figure 2. From figure 2а it is visible that cells differently react to virus infection at
different МOI. After infection at MOI 0,01, 0,001 and 0,0001 reduction of quantity of nitrite
in cultural medium was observed: there was a decrease in synthesis NO by cells (figure 2,
curves 2,3,4). At these MOI decrease in level of nitrite was 20-25 %. At an infection of cells
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