Biomedical Engineering Reference
In-Depth Information
Chapter 3
P
RODUCTION OF
N
ITRIC
O
XIDE BY
E
NDOTHELIAL
C
ELLS
I
NFECTED WITH
H
ERPES
S
IMPLEX
V
IRUS
T
YPE
1,
UNDER THE
I
NFLUENCE OF
I
NTERFERON
O. N. Scheglovitova,
1
Z. V. Kuropteva,
2
L. G.Nagler,
2
N. N. Skliankona,
1
N. A. Boldyreva,
1
L. M. Bayder
2
and O. L. Belaia
3
1
The Gamaleya Institute Epidemiology and Microbiology, Moscow, Russia
2
Emanuel Institute of Biochemical Physics,
Russian Academy of Sciences, Moscow, Russia
3
Moscow State Medical and Dental University, Moscow, Russia
A
BSTRACT
It was shown that recombinant IFN-α activates nitrite oxide production by primary
culture of human vascular endothelium and the level of effects depends on compound
concentrations. HSV-1 infection of endothelial cells at a MOI 0,1 resulted in fast
activation NO synthesis, NO production was decreased at a low MOI. IFN-α pretreatment
and following HSV-1 infection of endothelial cells resulted in reducing of NO production
at 24 hours, it was noted inhibition of virus reproduction in cells at the same time; at 48
hours NO synthesis by cells was activated and at the same time it was noted both
increasing of virus reproduction at these experiments and activation effect of rec. IFN-α
without virus infection at first experiments.
It was considered that the mechanism of process noted points out compose
multicomponent dependence of recombinant IFN-α effects, HSV-1 infection and NO
production by culture of human vascular endothelium. Possibly the mechanism indicates
pleiotropic intracellular transduction pathways influenced to iNOS expression in
endothelial cells.
Kaywords:
Nitric oxide, endothelial cells, interferon alpha, herpes simplex virus type 1
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