PRODUCTION OF THERMOSTABLE DNA POLYMERASE SUITABLE FOR WHOLE-BLOOD POLYMERASE CHAIN REACTION - Biochemistry and Biotechnology: Research and Development - page 5

engineered chimeric enzyme SSO7D-Klen Taq DNA polymerase may withstand high whole
blood concentrations (up to 20%, v/v) and may be applied as a constituent of analytical test-
kits in clinical-laboratory investigations.

A CKNOWLEDGMENTS

The work was supported by a grant from the Belarus State Research Program
«Fundamental Basics of Biotechnologies».

R EFERENCES

[1] R. K. Saiki, D. H. Gelfand, S. Stoffel, S .J. Scharf, R. Higuchi, G. T. Horn, K. B.
Mullis, H. A. Erlich: Science , 239 (4839), 487 (1988).
[2] P G. Lantz, W. Abu al-Soud, R. Knutsson, B. Hahn-Hagerdal, P. Radstrom: Biotechnol.
Annu. Rev ., 5, 87 (2000).
[3] W. Abu al-Soud, P. Radstrom: J. Clin. Microbiol ., 39 (2), 485 ( 2001).
[4] B. Y. Qu, Z. Y. Wu, X. X. Tian, K. Chen, F. Fang: Anal. Bioanal. Chem. , 389 (5), 1499
(2007).
[5] Y. Wang, D. E. Prosen, L. Mei, J. C. Sullivan, M. Finney, P. B. Vander Horn: Nucleic
Acids Res ., 32 (3), 1197 (2004).
[6] M. B. Kermekchiev, L. I. Kirilova, E. E. Vail, W. M. Barnes: Nucleic Acids Res ., 37
(5), e40 (2009).
[7] A. R. Pavlov, G. I. Belova, S. A. Kozyavkin, A. I. Slesarev: Proc. Natl Acad. Sci. USA ,
99 (21), 13510 (2002).
[8] S. Sun, L. Geng, Y. Shamoo: Proteins , 65 (1), 231 (2006).
[9] D. A. Benson, I. Karsch-Mizrachi, D. J. Lipman, J. Ostell, D. L. Wheeler: Nucleic
Acids Res ., 36 (Database issue), D25 (2008).
[10] G. Wu, J. B. Wolf, A. F. Ibrahim, S. Vadasz, M. Gunasinghe, S. J. Freeland: J.
Biotechnol ., 124 (3), 496 (2006).
[11] R. M. Bertina, B. P. Koeleman, T. Koster, F. R. Rosendaal, R. J. Dirven, H. de Ronde,
P. A. van der Velden, P. H. Reitsma: Nature , 369 (6475), 64 (1994).