CLONAL MICROPROPAGATION ENDEMIC OF THE NORTHERN CAUCASUS MENTHA LONGIFOLIA (L.) HUDS - Biochemistry and Biotechnology: Research and Development - page 178

with unknown vectors. This indicates the need for a system improvement of important species
of viral pathogens. In the literature, known investigation on improvement of mints from a
viral infection by means of meristem culture in vitro . The methods for adventitious stem
formation [2], direct regeneration from somatic tissues [3]. Tissue culture method has been
successfully used to preserve rare varieties [1]. On the Black Sea city of Sochi grow several
species of the family Lamiaceae. Including endemic and endangered species Mentha
longifolia (L.) Huds. [4]. This is a perennial herb that has a creeping rhizome. Height 0,5 - 1,0
m under favorable conditions can reach 1.5 m high aromatic plant. Leaves paired, opposite,
the stem square. Lanceolate leaves 45-100 mm long and 20 mm in width. The leaves are
pubescent. Given the economic importance of this species, we conducted a study on the
development of a technique of introducing the culture and in vitro micropropagation of this
species. Biological material for research was collected near the village of Krasnaya Volya
Adler district of Sochi. Selected plants were grown in a greenhouse. We used the young
shoots. Nodal segments (0,5-1,0 cm long) were sterilized in a solution of 1.05% sodium
hypochlorite with Tween-20 (few drop) for 20 min and then washed with sterile distilled
water. Apical stem tips (0,2-0,3 mm) were then placed on medium Van-Hoof [4]. Four to five
weeks after initial culture, shoots were selected for our regeneration experiment. These
plantlets were cultured in the test tubes containing Murashige and Skoog (MS) basal medium
[5] and maintained at 22±2°C and 16 h photoperiod. The MS basal medium contained of MS
salts, 100 mg L -1 myo-inositol, 0.2 mg L 1 thiamine HCl, 0.1 mg L -1 pyridoxine HCl, 0.2 mg
L -1 ascorbic acid, 0,5 mg L -1 nicotinic acid, 20 g L -1 sucrose, and 0.6% cell culture reagent
agar. Medium adjusted to pH 5.8 before autoclaving. Received sterile culture (figure 1) are
grown in a growth chamber. To develop methods of increasing the multiplication factor and
non-sterile rooting cuttings vitro (figure 2). Pretreated in 0.003% solution of NAA micro root
cuttings from tubes rooted in vermiculite in plastic
Figure 1. Shoots, regenerated of Mentha longifolia from shoot apical meristem explant with (a) - BAP /
NAA (0.2 mg / l / 0.2 mg / l),(b) - BAP / NAA (1.0 mg / l / 0.2 mg / l).