FILAMENTOUS FUNGI OF WASTE BIOMASS OF HIGHER WATER PLANTS - Biochemistry and Biotechnology: Research and Development - page 162

We studied endoglucanase activity of filamentous fungi cultivated on Mandels and Farid
media with filter paper in comparative aspect (table 1). Farid medium earlier was successfully
used for some protoplast cultures of micromycetes [7]. It is optimal for submerged cultivation
and production of cellulolytic enzymes.
Determination of endoglucanase activity is the most specific and precise index for our
needs. The maximum endoglucanase activity was observed at strain Aspergillus terreus.
Cultivation of micromycetes revealed that in all variants the stable increase of endoglucanase
activity is observed on media with natural substrates (waste HWP biomass) (table 2).
Study of development of microorganisms on nutrient media with waste HWP biomass
revealed that presence in medium of organic nitrogen shortens lag-phase at selected producers
of cellulase and induces increased biosynthetic activity of filamentous fungi, which allows to
reach maximum indices on 4 th day of cultivation.
Based on preliminary evaluation of cellulolytic properties of selected cultures it may be
assumed that the higher spectrum of cellulase enzymes is probably characteristic for them.
Obtained results testify possibility of receipt of active cultures of micromycetes for
application in biofuel production.

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