Biomedical Engineering Reference
In-Depth Information
M
ETHOD OF
E
XPERIMENT
Determination of the total content of endogenous antioxidants (AO) in seeds of the
aforementioned cultures and sprouts was performed by an express method of initiated
oxidation of hydrocarbon - cumene, developed in the Institute of Chemical Physics RAS. It
enables to determine the mechanism of antioxidants (AO) of complex composition, their
concentration and the inhibitor constant (K
7
). The method is based on the laws of a chain
free-radical oxidation mechanism, when matter containing AO introduced in a model reaction
reacts with an active center (peroxide radical RO
2
). The reaction slows down due to
disruption of the chain reaction when the AO reacts with RO
2
. The induction period (τ) can be
determined from the braking time of the oxidation reaction and the AO concentration in the
investigated preparation is calculated. The period of induction and the quantity of oxygen
consumed
O
2
are determined from the following formulas /1-3/.
τ =
2[InH]
0
Wi
(1)
[RH]
= -
k
3
ΔO
2
(2)
k
7
ln (1
_
t/τ)
W
i
- the initiating rate, is calculated from the formula
W
i
= 6.8
.
10
-8
[AIBN] mole / l
.
s ,
(3)
where [AIBN] (AZO-bis-ISOBUTYRONITRILE) is the initiator concentration in mg per ml
of hydrocarbon; RH - hydrocarbon and InH - antioxidant.
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