THE DESTRCTION OF THE COMPOSITIONS BASED ON CHITOSAN COPOLYMERS WITH ACRYLIC MONOMERS BY MICROMYCETES - Biochemistry and Biotechnology: Research and Development - page 137

chitosan as in all the test the chitosanase activity increases meanwhile their synthetical part
remains intact. Does it mean that the synthetical part is not involved in the destruction
process? We can suppose that its destruction will take place later when chitosan is totally
destroyed.
Table 2. The resistance of some polymers to micromycetes (associative culture, State
Standard 9.049-91)

The fungi resistance
in marks (method 1)

Polymer The estimation of the fungi resistance

Chitosan 5 Not resistant

Polymethylacrylate 1 Resistant

Polyacrylamide 3 Not resistant

Polyacrylonitril 2 Resistant

Table 3. Exoferments activity of Aspergillus terreus during the process of the
destruction of the polymers

Activity
Experiment

Sample Ferments

Control

Chitosanаse
(glycose mg /protein mg ×hour)

708,00 13,40

Grafted copolymer
chitosan : АА
[АА]/[ glycosamine]= 9,5
mole/base-mole

Proteinase acidic (мcМ tyrosine/mg
protein×hour)

trace trace

Proteinase alkaline (мcМ of tyrosine/mg of
protein×hour)

432,00 Trace

Chitosanаза
(mg of glycose/mg of protein×hour)

893,00 54,00

Grafted copolymer
chitosan : АН
[АН]/[ glycosamine]= 3
mole/base-mole

Peroxidase
(conventional unit, c.u.)

25,02 10,32

Catalase
(c.u.)

91,23 24,78

р<0,05.

Table 4. Exoferments activity of Penicillium сyclopium during the process of the
destruction of the polymers

Activity
Experiment

Sample Ferments

Control

Chitosanаза
(mg of glycose/mg of protein×hour)

Grafted copolymer chitosan :
МА
[МА]/[ glycosamine]= 1,9
mole/base-mole

957,00 152,00

Lipase
(ml of 0,05N NaOH/mg of
protein×hour)

1,20 1,98

Chitosanаза
(mg of glycose/mg protein×hour)

191,00 23,00

Block-copolymer chitosan :
МА
[МА]/[ glycosamine]= 2,5
mole/base-mole

Lipase
(ml of 0,05Н NaOH/mg protein×hour)

3,45 6,46

р<0,05.