Biomedical Engineering Reference
In-Depth Information
Chapter 11
A
DSORPTION
B
EHAVIOUR OF
5-M
ETHYLRESORCINOL AND
I
TS
M
IXTURES WITH
L
YSOZYME AT
A
IR
-W
ATER
I
NTERFACE
E. I. Martirosova
*
and I. G. Plashchina
Emanuel Institute of Biochemical Physics RAS, Moscow, Russia
A
BSTRACT
The adsorption kinetics of 5-methylresorcinol (MR) at water-air interface after 2 and
24 hours incubation of MR solutions in 0.05M phosphate buffer with pH 7.4 at 25
O
C was
studied using a dynamic drop tensiometry via drop shape analysis techniques. The
influence of methylresorcinol on adsorption of a model protein (hen egg lysozyme) was
studied at fixed protein concentration 3.4 10
-6
M and varying methylresorcinol
concentration.
I
NTRODUCTION
Nowadays there is a strong trend towards investigation of structure and functions of
microbial low molecular extra-cellular metabolites, represented in a number of bacteria and
yeast cells by alkylhydroxybenzenes (AHB). Alkylresorcinols in particular, inducing
transition of microbial cells into a hypo-metabolic (anabiotic) state [Bespalov et al., 20000;
Kolpakov et al., 2000], are capable also to affect the functional activity, operational stability
and substrate specificity of some enzymes [Petrovskii et al., 2009; Martirosova et al., 2004].
Another direction of using AHB, having a great practical importance, is modification of
enzymes (lysozyme, for example) to increase its resistance to action of microorganisms with
maintenance of its activity. Lysozyme is widely used both in medicine when treating
infections diseases and in the food industry to prevent bacterial infection of products. In this
connection, acquisition of resistance to lysozyme by microorganisms is becoming an
Search WWH ::
Custom Search