Biomedical Engineering Reference
In-Depth Information
method at parai n-impregnated graphite electrode for clenbuterol (CLB)
sensing, which was characterized by means of i eld emission scanning
electron microscopy (FE-SEM), X-ray photoelectron spectroscopy spec-
tra (XPS), infrared spectra (IR) and electrochemical techniques to coni rm
the formation [75]. h e molecular imprinted i lm modii ed electrode was
successfully applied to the determination of CLB with a reliable result. In
optimum conditions, CLB at concentrations of 0.3-50.0 μM could be deter-
mined with a detection limit of 0.01 μM (3r). Determination of CLB in
practical samples of pork liver showed good recovery. Lu
et al.
reported
a technique of forming nanoi lms of poly-3-aminophenylboronic acid
(pAPBA) on the surfaces of polystyrene (PS) microbeads for proteins
(papain and trypsin) in aqueous [76]. Papain was chosen as a model to study
the feasibility of the technique and trypsin as an extension. h e results show
that pAPBA formed nanoi lms (60-100 nm in thickness) on the surfaces
of PS microbeads. h e specii c surface area of the papain-imprinted beads
was about 180m
2
g
-1
and its pore size was 31 nm. h ese imprinted micro-
beads exhibit high recognition specii city and fast mass transfer kinetics.
h e specii city of these imprinted beads mainly originates from the spatial
ef ect of imprinted sites, because the protein-imprinted sites were located
at, or close to, the surface, the imprinted beads have good site accessibil-
ity toward the template molecules. h e facility of the imprinting protocol
and the high recognition properties of imprinted microbeads make the
approach an attractive solution to problems in the i eld of biotechnology.
12.3
Imprinted Materials at Nanoscale
12.3.1 Imprinted Nanoparticle
Recently, Kobra
et al.
reported the synthesis of nanoparticles of MIPs were
by precipitation polymerization method using glucose as a template mole-
cule [77]. Experimental data based on uniform design were analyzed using
artii cial neural network to i nd the optimal condition. h e results showed
that the binding ability of nanoparticles of MIPs prepared under optimum
condition was much higher than that of the corresponding nonimprinted
nanoparticles (NIPs).
Behbahani
et al.
describes the preparation of new Pb(II)-imprinted
nanostructured polymeric particles using 2-vinylpyridine as a functional
monomer, ethylene glycol dimethacrylate as the cross-linker, 2,2'-azobi-
sisobutyronitrile as the initiator, diphenylcarbazone as the ligand, ace-
tonitril as the solvent, and Pb(NO
3
)
2
as the template ion, through bulk
