Biomedical Engineering Reference
In-Depth Information
BHb
DA
Extraction
Polymerization
Rebinding
SiNW
Figure 12.23
Schematic of the preparation of MIP for Bovine Hemoglobin (BHb)
recognition [53].
In 2012, Chen
et al.
employed silicon nanowires as the reinforcement
material in protein molecular imprinting with dopamine as the monomer
and BHb as the template molecule (Figure 12.23). In the experiments, the
imprinted nanowires showed fast adsorption kinetics (taking up 75% of the
equilibrium amount during only 5 min), signii cant selectivity and large
binding capacity (213.7 mg g
-1
) for the template protein. Furthermore, the
stability and regeneration were also investigated, which indicated that the
imprinted nanowires had outstanding reusability.
h e electrospinning method was also employed for the preparation
of imprinted nanoi bers. For example, Kim
et al.
prepared an estrone-
imprinted polyimide nanoi ber mat using an electrospinning method [54].
h e diamine monomer-template complex was synthesized by the reaction
of the diamine monomer having an isocyanate group and estrone (tem-
plate) having a phenol moiety, in which the template was attached to the
monomer via a thermally reversible urethane bond. A poly(amic acid)
was synthesized by polymerization of the diamines (1:19 mole ratio of
the diamine monomer-template complex to 4,4 -oxydianiline) and pyro-
mellitic dianhydride in N,N-dimethylformamide and the reaction solution
was used for electrospinning (Figure 12.24). h e poly(amic acid) i bers
were thermally imidized and then heated in 1,4-dioxane in the presence of
water to remove the template molecules. h e imprinted polyimide nano-
i bers showed specii c recognition ability and fast kinetic adsorption for
estrone.
12.2.1.8
Carbon Nanotubes (CNTs)
Carbon nanotubes can be visualized as a sheet of graphite that has been
rolled into a tube. Unlike diamond, where a 3D diamond cubic crystal
structure is formed with each carbon atom having four nearest neighbors
