Biomedical Engineering Reference
In-Depth Information
h e imprinted BPA molecules were removed by a simple thermal treat-
ment to generate the imprint-removed material, MIP-ir-AuNPs, with the
desired recognition sites that could selectively rebind the BPA molecules.
h e morphological and polymeric characteristics of MIP-ir-AuNPs were
investigated by transmission electron microscopy and Fourier-transform
infrared spectroscopy. h e results demonstrated that the MIP-ir-AuNPs
were fabricated with a 2-nm MIP shell layer within which abundant amine
groups were generated. h e rebinding kinetics study showed that the MIP-
ir-AuNPs could reach the equilibrium adsorption for BPA within 10 min
owing to the advantage of ultrathin core-shell nanostructure. Moreover, a
linear relationship between SERS intensity and the concentration of BPA
on the MIP-ir-AuNPs was observed in the range of 0.5-23 22.8 mg/L, with
a LOD of 0.12 mg/L.
Similarly Du
et al.
also reported a core-shell composite of AuNPs and
SiO
2
molecularly imprinted polymers (AuNPs@SiO2-MIPs) through sol-
gel technique and applied as a molecular recognition element to construct
an electrochemical sensor for determination of dopamine [42]. Compared
with previous imprinting recognition, the main advantages of this strat-
egy lie in the introduction and combination of AuNPs and biocompatible
porous sol-gel material (SiO
2
) (Figure 12.16). h e template molecules were
i rstly adsorbed at the AuNPs surface due to their excellent ai nity, and
subsequently they were further assembled onto the polymer membrane
through hydrogen bonds and p-p interactions formed between template
PTMOS, TMOS
DPV
GCE
GCE
E/V
Gold nanoparticles
PVP
Au@SiO
2
-MIPs
(before elution)
Template (DA)
Figure 12.16
Preparation procedure of the Au@SiO2-MIPs (Reproduced with permission
from [42]).
