Biomedical Engineering Reference
In-Depth Information
according to this newly developed approach for surface protein imprinting
over nanoparticles. h e rebinding experiments coni rmed that the intro-
duction of the carboxyl groups could remarkably improve the imprinting
ef ect in relation to a signii cantly increased imprinting factor and specii c
rebinding capacity. Moreover, in contrast to the harsh template removal
conditions required for the covalent template coupling approach, the tem-
plate removal during the imprinted particle synthesis as well as desorption
at er rebinding could be mildly achieved via washing with salt solution.
Chemical nanosensors with a submicrometer core-shell composite
design, based on a polymer core, a MIP shell for specii c analyte recogni-
tion, and an interlayer of gold nanoparticles for signal amplii cation, were
also reported by Bompart
et al.
[37]. Surface-enhanced Raman scatter-
ing (SERS) measurements on single nanosensors yield detection limits of
10
−7
M for the
β
-blocker propranolol, several orders of magnitude lower
than on plain MIP spheres.
Gültekin
et al.
have proposed a novel thiol ligand-capping method with
polymerizable methacryloylamido-cysteine (MAC) attached to gold-
silver nanoclusters, reminiscent of a self-assembled monolayer, and have
reconstructed surface shell by synthetic host polymers based on molec-
ular imprinting method for recognition [38]. In this method, methacry-
loylamidoantipyrine-terbium (MAAP)
2
-Tb(III) has been used as a new
metal-chelating monomer via metal coordination-chelation interactions
and dipicolinic acid (DPA) which is main participant of Bacillus cereus
spores used as a model. Nanoshell sensors with templates give a cavity that
is selective for DPA. h e DPA can simultaneously chelate to Tb(III) metal
ion and it into the shape-selective cavity. h us, the interaction between
Tb(III) ion and free coordination spheres has an ef ect on the binding abil-
ity of the gold-silver nanoclusters nanosensor. h e binding afinity of the
DPA imprinted nanoclusters has been investigated by using the Langmuir
and Scatchard methods, and the respective afinity constants determined
were found to be 1.43×10
-4
and 9.1×10
-6
mol L
-1
.
Recently Chen
et al.
have reported a sensitive and selective electrochem-
ical sensor for metronidazole (MNZ) [39]. h e core-shell metronidazole-
m-MIP was synthesized and then attached to the surface of magnetic glassy
carbon electrode (MGCE) with the help of magnetic force in order to pre-
pare m-MIP/MGCE sensor [39]. h e two very popular techniques of CV
and EIS have been applied to investigate the performance of the obtained
imprinted sensor. Various factors known to af ect the response behavior of
the MMIP/MGCE electrode were studied and optimized. h e imprinted
sensor exhibits high recognition ability and ai nity for MNZ in com-
parison with the nonimprinted one. In addition, the MMIP/MGCE also
