Biomedical Engineering Reference
In-Depth Information
MPS
TEOS
NH
3
H
2
O
MBAAm, APS,
TEMED
Fe
3
O
4
Fe
3
O
4
@SiO
2
Modififed-Fe
3
O
4
@SiO
2
Fe
3
O
4
@SiO
2
@MIPs
Lysozyme
AAm
MAA
Rebinding
protein
Removal
protein
Sample
Luminiuo,
CTMAB,
K
3
[Fe(CN)
6
]
Nacl
solution
Phosphate
buffer
Phosphate
buffer
96-well plate
Chemiluminescence analyzer
Magnet
Eluting
Washing
Loading
Conditioning
Figure 12.10
A schematic representation of Fe
3
O
4
@SiO
2
@MIPs preparation and CL
detection of lysozyme (Reproduced with permission from [29]).
sites to induce imprinting polymerization (Figure 12.11). h e absorp-
tion capacity results showed that the molecularly imprinted polymers
had an excellent combining ai nity, recognition selectivity and fast
kinetics. Furthermore, the molecularly imprinted polymers were suc-
cessfully used as absorbent of dispersive solid-phase extraction coupled
with high-performance liquid chromatography to determinate trace
estriol and estradiol in milk tablets. h e high recoveries yield of 89.1-
93.5% were achieved with the relative standard deviations less than
9.4%. So, the molecularly imprinted polymers are ef ective absorbents
for the separation and enrichment of oestrogens in the complex matri-
ces samples.
Similarly, Peng
et al.
reported the preparation of metsulfuron-methyl
(MSM)-imprinted polymer layer-coated silica nanoparticles toward
analysis of trace sulfonylurea herbicides in complicated matrices [31]. To
induce the selective occurrence of surface polymerization, the polymeriz-
able double bonds were i rst grat ed at the surface of silica nanoparticles
by the silylation using 3-(Methacryloxy) propyl trimethoxysilane (MPTS)
(Figure 12.12). At erwards, the metsulfuron-methyl templates were
