Pluripotent Stem Cells from the Postnatal Testis: Unlocking the Potential of Spermatogonial Stem Cells - Male Germline Stem Cells Developmental and Regenerative Potential - page 42

2.9 Addendum

Since this chapter was originally prepared, two recent publications merit brief men-
tion. First, Kuijk et al. ( 2010 ) have demonstrated that NANOG is present at the
level of mRNA and protein in the adult testis of various species, particularly in
meiotic and post-meiotic germ cells but also at lower levels in spermatogonia
(Kuijk et al. 2010 ). Second, using an experimental approach, Ko et al. ( 2010 ) have
strongly questioned the results of Conrad et al. ( 2008 ) and suggested that the cells
produced in the latter study were not actually pluripotent but rather more similar to
fibroblasts (Conrad et al. 2008 ; Ko et al. 2010 ).

Acknowledgments This work was supported by the Howard Hughes Medical Institute, the
Ansary Center for Stem Cell Therapeutics, and National Heart, Lung and Blood Institute grants
HL075234, HL059312, and HL084936 (S.R.), and the ASCO Young Investigator Award (M.S.).
Marco Seandel is a Stanley and Fiona Druckenmiller New York Stem Cell Foundation Fellow.

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