Biomedical Engineering Reference
In-Depth Information
Chapter 11
Translating Spermatogonial Stem Cell
Transplantation to the Clinic
Brian P. Hermann and Kyle E. Orwig
Abstract
The spermatogenic lineage is maintained by a pool of spermatogonial
stem cells (SSCs) that balance self-renewing and differentiating divisions to meet
the biological demand of the testis. In animal models (mice, rats, goats, pigs,
and dogs), SSCs transplanted into infertile recipient testes exhibit the remarkable
potential to regenerate spermatogenesis. Tremendous progress has been made
towards applying SSC transplantation techniques to primates (human and nonhu-
man), which may have applicability in the human fertility clinic. This chapter
will review the approaches used to isolate and preserve donor SSCs from primate
testes, generate infertile recipient nonhuman primates for transplantation studies,
transplant donor SSCs into primate testes, and evaluate transplant outcomes.
In addition, we discuss some of the clinical considerations for future application of
these techniques in patients.
Keywords
Spermatogonial stem cells
•
Primates
•
Male infertility
•
Spermatogenesis
•
Transplantation
B.P. Hermann (
*
)
Department of Obstetrics, Gynecology and Reproductive Sciences,
Magee-Womens Research Institute, University of Pittsburgh School of Medicine,
204 Craft Avenue, Pittsburgh, PA 15213, USA
and
Department of Biology, The University of Texas at San Antonio
e-mail: hermannbp@upmc.edu
K.E. Orwig (
*
)
Department of Obstetrics, Gynecology and Reproductive Sciences,
Magee-Womens Research Institute, University of Pittsburgh School of Medicine,
204 Craft Avenue, Pittsburgh, PA 15213, USA
e-mail: orwigke@upmc.edu
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