Testicular Xenografting - Male Germline Stem Cells Developmental and Regenerative Potential - page 215

Human (xenologous) Adult (intact and

disturbed
spermatogenesis)

No None - Donors with obstructive azoospermia,

hypospermatogenesis, or SCO: complete
atrophy and hyalinization, no germ
cells- Donor with testicular cancer: mostly
SCO tubules, some A-spermatogonia

Schlatt et al.
( 2006 )

- Transsexual donors post estradiol treatment:

some A- and few B-spermatogonia

Rhesus monkey
(autologous)

Juvenile (prepubertal,
13 months)

Ye s Hormone

injections

Spermatocytes after several weeks of hCG
stimulation

Orwig and
Schlatt
( 2005 )

Rhesus monkey
(xenologous)

Juvenile (prepubertal,
13 months)

No No - After 2 months: sp ermatogonia

only- After 4 months: spermatocytes in 30%
and spermatids in 3% of seminiferous tubules

Honaramooz
et al.
( 2004 )

- After 7-12 months: complete

spermatogenesis and sperm in 4% of
seminiferous tubules

- ICSI with sperm from xenografts and in vitro

matured eggs led to blastocyst development

Marmoset
(xenologous)

Neonate, infantile and
juvenile (0, 1, 3, 7
months)

No Co-grafting

of hamster
tissue

- 30% complete atrophy of tissue Wistuba et al.

( 2004 ) - 60% SCO tubules in surviving grafts

- A-spermatogonia in 40% of surviving grafts

- No spermatocytes or spermatids

- No benefit through co-grafting of hamster

testes

(continued)