Testicular Xenografting - Male Germline Stem Cells Developmental and Regenerative Potential - page 214

Table 10.2 (continued)

Primate species and
type of grafting

Age of donors and status
of spermatogenesis

Cryopreservation
before grafting

Treatment
of recipient

Status of grafted testicular tissue and
spermatogenic activity in grafted tissue References

Human (xenologous) Fetal (20/26 weeks of

gestation)

No None - Integrity of the testicular tissue in grafts was

normal and changed from fetal to
pre-pubertal developmental status 116
and 135 days post-grafting

Yu et al.
( 2006 )

- Germ cells were mostly located at the

basement membrane

- Seminiferous tubules increased in diameter

and presented a small lumen

Rhesus monkey
(xenologous)

Juvenile (16/19 months) No Busulfan

injections

- Integrity of the testicular tissue in grafts was

normal 28 and 32 weeks post-grafting

Jahnukainen
et al.
( 2006a ) - Most advanced germ cell types were

B-spermatogonia, spermatocytes and at 32
weeks also spermatids

- Busulfan treatment of mice reduced germ cell

numbers in xenografts

Marmoset (autologous) Infantile (4 weeks) No None - 5 of 16 grafts were recovered 17 months

post-grafting

Wistuba et al.
( 2006 )

- 35% of seminiferous tubules were SCO

- 30/25% contained spermatogonia/

spermatocytes

Human (xenologous) Adult (intact

spermatogenesis)

No None - Complete atrophy and sclerosis in 60% of the

grafts- Complete SCO in 10-20% of grafts

Geens et al.
( 2006 )

- Some A-spermatogonia in 20% of grafts

- No difference between mouse recipients

(SCID-NOD versus Swiss Nude)

- More atrophy with time (40% at 30-119 days

versus 70% at 120-195 days post-grafting)