Biomedical Engineering Reference
In-Depth Information
Human (xenologous)
Immature (2-12 years)
crypt-orchid
Ye s
None
-
Three weeks after thawing and grafting,
testicular tissue showed only minor
degenerative changes
Wyns et al.
(
2007
)
-
Spermatogonial numbers are reduced
to 15% compared to fresh tissue after
cryopreservation and grafting
-
Spermatogonia and Sertoli cells were more
frequently proliferating in grafts compared to
fresh tissue
Rhesus monkey
(xenologous)
Juvenile (18-21 months)
Ye s
None
-
50-70% of testicular grafts were recovered
from fresh tissue showing good structural
preservation 3 and 5 months post-grafting
Jahnukainen
et al.
(
2007a
)
-
Depending on the type of cryoprotectant
and details of the protocol, less grafts were
recovered following cryopreservation,
thawing, and grafting
-
Structural preservation of thawed grafts was
good
-
Most advanced germ cells were
B-spermatogonia after 3 months and
spermatocytes after 5 months
-
Spermatogonial numbers were better
maintained in tissue cooled for 24 h when
compared to cryopreservation with DMSO
(continued)
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