Biomedical Engineering Reference
In-Depth Information
Fig. 8.5
A live imaging study of GFP-labeled Ngn3
+
A
undiff
upon transition into differentiating
spermatogonia. (
a
) Selected frames of a live imaging study of spermatogonia upon A
undiff
-to-A
1
transition. The elapsed time is indicated in each panel in hours. Before transition (0-12 h), A
undiff
preferentially localized to the area adjacent to the blood vessels (seen as a
black line
). Upon tran-
sition into A
1
, two chains of eight-cell cysts (A
al-8
; indexed in
yellow
and
orange
) migrated from
this position to spread all over the basal compartment of the tubule (by ~36-60 h), followed by
synchronous divisions. See (Yoshida et al.
2007b
) for details. (
b
-
e
) Examples of the vasculature-
proximal localization of A
undiff
(
arrowheads
), more characteristically to their branch points.
Modified from Yoshida et al. (
2007b
)
Fig. 8.6
Schematic of the vasculature-associated niche for A
undiff
. (
a
) A schematic of the proposed
vasculature-associated microenvironmental niche for A
undiff
(and possibly the stem cells), shown in
red
. Important components include blood vessels, interstitial cells, myoid cells, Sertoli cells, and
the basement membrane. Sertoli and myoid cells in this region might be “specialized,” as sche-
matically shown in
deeper blue
and
green
, respectively. (
b
) The possible controlling pathways of
A
undiff
/stem cells, shown in
arrows 1-5
.
Dotted arrows
indicate a possible indirect effect from the
blood vessels/interstitium. Cells are not shown proportionally in size. See text for details
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