Spermatogonial Stem Cell Transplantation and Culture - Male Germline Stem Cells Developmental and Regenerative Potential - page 120

differential plating with plastic and collagen matrices (Hamra et al. 2008 ). Recently,
it was reported that hamster SSCs can be cultured for long periods (Kanatsu-
Shinohara et al. 2008a ). The culture system for these cells is very similar to that for
other rodents.

5.3.4.2 Non-rodent

Considerable effort has been devoted to the development of non-rodent SSC cul-
ture; however, confirmation of success is lacking. Recent reports exist describing
SSC culture for bovine (Aponte et al. 2008 ) and porcine SSCs (Luo et al. 2006 ).

5.4 Summary

The development of techniques to transplant and culture the SSC have the potential
to revolutionize reproductive management of various species, including humans,
livestock, and endangered species. Furthermore, combined applications of these
techniques are essential for amplification of SSCs and the efficient development of
transgenic animals. Much progress has been made in the culture and transplantation
of rodent SSCs; yet, progress in developing these techniques in other species of
economical or societal value is minimal. However, translation of these techniques
to species such as livestock and humans will open many doors for novel methods
of reproductive management and are currently the next frontier in the study of SSC
biology.

Acknowledgments We would like to thank James Hayden for assistance in preparation of fig-
ures. Financial support was provided by the National Institute of Health (HD044445 and
HD052728) and the Robert J Kleberg Jr, and Helen C Kleberg Foundation.

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