Environmental Engineering Reference
In-Depth Information
0.8
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0
MOS1 _Channel max
MOS2_Channel max
MOS3_Channel max
ScCell1_Channel max
ScCell2_Channel max
Figure 13.5b. Syringe 2: sludge with TYG(1:100), gas mixture 2/3 N
2
and 1/3 air. See Fig. 13.5a for
explanations.
1
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0.8
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0.5
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0.1
0
MOS1 _Channel max
MOS2_Channel max
MOS3_Channel max
ScCell1_Channel max
ScCell2_Channel max
Figure 13.5c. Syringe 3: sludge withTYG (1:10),
Klebsiella mobilis
1:1000, gas mixture 2/3 N
2
and 1/3 air.
See Fig. 13.5a for explanations.
communities were able to exploit the available organic compounds within a longer time range,
whereas a rapidly growing and metabolized production strain was more effectively depriving
some essential components from the medium.
Interestingly, the natural microbial culture was shown to contain remarkable amounts of kleb-
siellas as determined by the Chromagar™mediumafter 9.5 hours of PMEU cultivation.
Klebsiella
mobilis
strain (butanediol fermenting bacterium) was added as pure inoculant culture to the cir-
culated washing liquid (Fig. 13.5a-d). Both in case of naturally “fermented” and in inoculated
fluid the proposition of the added TYG medium clearly influenced the outcome as measured by
the volatile compounds. In the determination from the raw washing liquid, no klebsiellas could
be verified, and the number of
E. coli
cells was around 1000/mL. Moreover, during these 9.5
hours the non-inoculated substrate had been producing 134
g/mL of 2,3-butanediol, whereas
the sample from the
K. mobilis
laboratory strain containing culture at the same time point resulted
in 61
µ
g/mL of the butanediol. This preliminary experimentation shows that such substrate as car
washing liquid could form a solution for diluting organic wastes, and it also contains a variety
of microorganisms capable of starting the production of biochemicals. It is noteworthy that this
µ
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