Environmental Engineering Reference
In-Depth Information
Figure 13.3. PMEU Spectrion®. All PMEU models are innovated in Finnoflag Oy. Some versions have
beenmanufactured by Samplion Oy. Both companies are fromKuopio and Siilinjärvi, Finland.
0
Syr 1
Syr 2
Syr 3
Syr 4
Syr 5
100
200
300
400
500
Figure 13.4. Growth of
E. coli
in PMEU Spectrion® (Samplion Oy and Finnoflag Oy, Kuopio, Finland)
using Colilert™ media (IDEXX Laboratories Inc., Maine, USA).
syringe 2, from syringe 2 to syringe 3 and so on. The gas flows from a syringe to the next one
as well as the final cell concentrations are presented in Table 13.2. Temperature was set at 37
◦
C.
E. coli
amount was about 2
×
10
4
cfu/mL in the beginning of the experiment in every syringe.
13.8.3
Anaerobiosis made efficient
Pasteur already proposed that the aerobic bacteria were consuming free molecular oxygen (O
2
)in
mixed cultures, which also gave the opportunity for the anaerobic bacteria to survive beneath the
aerobic flora. When
E. coli
was used for the purpose of oxygen removal, an E
h
value (oxidation-
reduction potential) of
−
440mV was achieved (Porter, 1948). Much higher values up to
+
50mV
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