Biomedical Engineering Reference
In-Depth Information
(a)
(b)
Image: 'BHK21 control2013, To pograph, 0.00[V] Bias, right-left
Image: 'BHK21 treated010, To pograph, 0.00[V] Bias, left-right
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Image: 'HT29 control004, To pograph, 0.00[V] Bias, right-left
Image: 'HT29 treated005, To pograph, 0.00[V] Bias, right-left
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FIGURE 10.4 AFM analysis of AgNPs treated BHK21 and HT29 cells. Untreated cells: (a) BHK21 (c)
HT29. AgNPs-treated cells: (b) BHK21 (d) HT29. (Reprinted with permission from Gopinath, P. et al. 2010.
Colloids. Surf. B. Biointerfaces . 77, 240-245.)
10.3.5 I n V Itro h eMolysIs t est
The aim of this test is to test the biocompatibility of nanoparticles. The results reveal the impact
of nanoparticle physicochemical characteristics on human red blood cells. Sayes et al. (2007) have
found that the incubation of amorphous- or crystalline-silica nanoparticles produce the hemolysis
of erythrocytes.
10.3.6 g eNotoxIcIty t estINg
Genotoxicity tests are conducted to identify potential genotoxic carcinogens and germ cell mutagens.
“Safe” nanotechnology can be established only when it is proven to have a nongenotoxic nature.
The single-cell gel electrophoresis assay (Comet assay) is a fundamental and sensitive technique
for the detection of DNA damage and repair at the level of the individual cell. It is widely applied
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