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(a)
(b)
(c)
(d)
Figure 3.6
Resolution.
4. Based on your answer to Question 3.2 and using Equation (3.6),
calculate the height equivalent to a theoretical plate (in units of
millimetres).
5. Based on your answers to Question 3.3 and using Equation (3.6),
calculate the height equivalent to a theoretical plate (in units of
millimetres).
6. A compound with a retention time of 6.3 min and a peak height of
624,352 (μV) has been assessed for peak asymmetry at (a) 10% of its
peak height to have a value for 'a' of 1.8 s and a value for 'b' of 2.2 s,
and (b) 5% of its peak height to have a value for 'a' of 2.0 s and a value
for 'b' of 2.5 s. Calculate the peak asymmetry using Equations (3.7)
a nd (3.8).
7. The separation of some compounds by gas chromatography with a
flame ionisation detector was done. On visual inspection, it appears
that two of the compounds may not be separated (i.e., resolved).
Compound A has a t
r
of 3.32 min and a peak width at its base of 6.5
s, while compound B has a t
r
of 3.51 min and a peak width at its base
of 7.9 s. Calculate the resolution of the peaks and hence determine
whether they are resolved or not using Equation (3.9).
Further Reading
Blumberg, L. M. 2010.
Temperature-programmed gas chromatography.
Chichester,
UK: John Wiley & Sons.
Fowlis, I. A. 1995.
Gas chromatography,
2nd ed.
Analytical chemistry by open learning.
Chichester, UK: John Wiley & Sons.
Grob, R. L., and E. F. Barry. 2004.
Modern practice of gas chromatography,
4th ed.
Hoboken, NJ: John Wiley & Sons.
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