Chemistry Reference
In-Depth Information
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Figure 8.22
Extracted ion (86 Da) chromatogram for acidic extract.
Again, a known standard of this metabolite would be run and retention times
and mass spectra compared.
Since an internal standard (Proadifen) was added to the blood sample
prior to extraction, it must also be identified in the blood sample. The molec-
ular ion for this compound is 86 Da; therefore, this ion can be extracted from
the TIC, as we have done previously (Figure 8.22).
The first two peaks on the chromatogram are the already identified fatty
acids; the mass spectrum for the third peak at 15.9 min (Figure 8.23) shows
the most abundant ion at 86 Da, which is what is expected for Proadifen. As
previously mentioned, a pure standard would be run for comparison.
On examining every peak on the original TIC (Figure 8.15) no other
drugs were found in the acidic extract. The same procedure was carried out
for the basic extract; however, only Proadifen (internal standard) was found.
This is expected since Proadifen is amphoteric.
8.4
Forensic Analysis of Fire Debris
In order for a fire to occur, several conditions must exist:
• A combustible fuel must be present.
• An oxidiser (such as the oxygen in air) must be available in sui-
cient quantity.
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