Agriculture Reference
In-Depth Information
FIGURE 5.8
(
See color insert.
).Heat.stress.induced.removal.of.the.
nptII
.marker.gene.on.
apple..Transgenic.apple.plants.containing.the.
nptII
.marker.gene.and.an.
Flp
.recombinase.gene.
between. two. FRT. recombination. sites. were. produced.. The. expression. of. the. Flp. recombi-
nase.is.controlled.by.a.heat-stress.inducible.promoter..Both.genes.(
nptII
.and.
Flp
).are.located.
between.the.CaMV.35S.promoter.and.the.
uidA
.marker.gene..In.transgenic.plants.containing.
nptII
.and.
Flp
.no.GUS.gene.expression.is.possible.(left),.because.the.CaMV.35S::
uidA
.reading.
frame.is.interrupted..The.reading.frame.is.restored.by.excision.of.
nptII
.and.
Flp
..GUS.expres-
sion.is.only.possible.in.cells.in.which.both.genes.have.successfully.been.removed.(right)..(The.
igure.was.kindly.provided.by.Katja.Herzog,.Julius.Kühn-Institut,.Federal.Research.Centre.
for.Cultivated.Plants.(JKI),.Institute.for.Breeding.Research.on.Horticultural.and.Fruit.Crops.
Dresden,.Germany;.unpublished.)
a. gene. transferred. from. a. crossable. species. with. its. native. introns. and. lanking.
regions,.such.as.native.promoter.and.terminator.in.a.sense.orientation..Such.“cisgenic”.
plants
11
.will.probably.be.sooner.accepted.than.GM.plants.containing.marker.genes.
and.genes.of.noncrossable.species.
REFERENCES
.
1.. Block,. G.,. Patterson,. B.,. and. Subar,. A.,. Fruit,. vegetables. and. cancer. prevention:.
A review.of.the.epidemiological.literature..
Nutrition and Cancer
,.18,.1,.1992.
.
2.. Ferro-Luzzi,.A..et.al.,.The.Mediterranean.diet.revisted:.Focus.on.fruit.and.vegetables..
International Journal of Food Science and Nutrition
,.45,.291,.1994.
.
3.. Morris,. D.M.,. Kritchevsky,. S.B.,. and. Davis,. C.E.,. Serum. carotenoids. and. coronary.
heart.disease:.The.lipid.research.clinics.coronary.primary.prevention.trial.and.follow-up.
study..
The Journal of the American Medical Association
,.274,.1439,.1994.
.
4.. Flachowsky,. H.. et. al.,. A. review. on. transgenic. approaches. to. accelerate. breeding. of.
woody.plants..
Plant Breeding
,.128,.217,.2009.
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