Agriculture Reference
In-Depth Information
Recently, functional screening has been used to comprehensively
investigate the genes involved in salt tolerance in mangroves (Table 3).
Functional screening of microorganisms that express heterologous cDNA
libraries is a powerful tool for identifying genes with specific functions,
independent of the regulation of their expression. Recombinant expression of
plant cDNAs in Escherichia coli has been used to successfully identify genes
that are involved in enhanced salt tolerance. Functional screening on medium
containing NaCl resulted in identification of the gene encoding allene oxide
cyclase (AOC, or ‗mangrin') as a salt-tolerance gene in Bruguiera sexangula
[58]. An unusual 70-amino acid sequence was shown to be essential for the
salt-tolerant phenotype.
Table 3. Salt tolerance-related genes identified in functional screening of
mangroves. Modified from Tada [57]
Mangrove
species
Salt-stress related
genes/proteins
Methods
Description
References
Plasma membrane intrinsic
protein
Myoinositol-1-phosphate
synthase
Gluthathione S-transferase
Manganese superoxide
dismutase
Calcium binding EF family
GTP binding proteins
107 salinity-tolerant
candidate genes were
identified. 65% have
not been reported to be
stress-related.
E. coli
functional
screening
Acanthus
ebracteatus
[63]
Ubiquinol-cytochrome C
reductase complex 7.8 kDa
protein
Small Ras-like GTP-
binding protein
GIGANTEA
Kef-type K + transport
systems
Aerobic-type carbon
monoxide dehydrogenase,
large subunit CoxL/CutL
homologs
SOS-response
transcriptional repressors
Putative senescence-
associated protein
Probable disease resistance
protein
LexA repressor
10 tentative salt-
responsive genes were
identified by SSH.
SSH and
bacterial
functional
screening
Bruguiera
cylindrica
[64]
29 cDNAs conferred
salinity tolerance to E.
coli.
Search WWH ::




Custom Search