Agriculture Reference
In-Depth Information
Table 4. (Continued)
Representationa
l difference
analysis of
cDNA
HSP (heat shock proteins)
ARF (ADP-ribosylation
factor)
Unknowns
Five up-regulated
and five down-
regulated genes
were isolated.
Kandelia
candel
[65]
Microarray analysis
revealed that 865 of
11,997 genes
showed significant
differential
expression under
salt and osmotic
stress.
Hormone response factors
Transporters
CaM-like proteins
Ca
2+
/H
+
exchanger
LEA (late embryogenesis
abundant protein)
Microarray
[39]
Bg70
β-lactamase
LTP4 (lipid transfer protein
4)
Phosphorylase family
protein
PBB2 (20S proteosome β
subunit B2)
ATUBC2 (ubiquitin-
conjugating enzyme 2)
Mitochondrial transcription
termination factor family
protein
A total of 537,635
sequences were
assembled and
annotated as
>13,000 distinct
gene models for
each species.
Within the top 20
most frequently
returned
annotations, there
were a number of
proteins unique to
each species.
Rhizopho
ra mangle
Transcriptomics
[55]
Unknown
Chloroplast DNA
MIOX1 (myo-inositol
oxygenase)
ATUBC2 (ubiquitin-
conjugating enzyme 2)
Mitochondrial transcription
termination factor family
protein
Heritera
littoralis
Transcriptomics
SSH was used to clone transcripts with elevated expression during salt
stress in the leaves of
A. corniculatum
[19]. Among the 577 ESTs obtained,
salt responsive expression of four transcripts, Δ
1
-pyrroline-5-carboxylate
synthase, Na
+
/H
+
antiporter and two plasma membrane intrinsic proteins, was
confirmed by real-time quantitative RT-PCR analysis. These results coincide
with proline accumulation in
A. corniculatum
under salt stress [19] and
suggest the involvement of Na
+
compartmentalization and proline synthesis in
salt tolerance mechanisms in
A. corniculatum
.
Because EST sequencing is a rapid and cost-effective method for
generating data on the coding capacity of genomes, it was performed on
