Chemistry Reference
In-Depth Information
TABLE 8.20 (continued)
Analysis of Biologically Important Compounds at Carbon Paste-Based Electrodes: Coenzymes, Enzymes, Proteins,
and Related Compounds
Analyte
Modiier
Carbon Paste
Techniques
Comments
Ref(s)
Lactate.
dehydrogenase
NADH.+.pyruvate
G/p.(solid)
LSV
Indirect.det..via.decreased.ox..of.NADH;.PBS.
0.15.M,.pH.7.4;.s:.biological.luids
[1313]
Lysozyme
IgG.(blocking).+.anti-lysozyme-
aptamer.+.lysozyme
SWV
Increased.immobilized.lysozyme.causes.decrease.
of.guanine.or.adenine.ox..of.aptamer;.ABS.
0.02.M,.pH.5
[2896]
Neuropathy.target.
esterase
Tyrosinase.+.1-methoxyphenazine.
methosulfate
G/po
CV
Biosensor;.determine.neuropathy.target.esterase.
activity.via.phenol.from.phenyl.valerate;.PBS.
0.05.M.+.0.1.M.NaCl,.pH.7.0;.LOD.25.nM;.
s: NTE.assay.in.hen.brain.and.blood
[2125,2776]
Neuropathy.target.
esterase
Tyrosinase
G/po
FIA.(−0.15.V.vs..SSE)
Biosensor;.determine.neuropathy.target.esterase.
activity.via.phenol.from.phenyl.valerate;.PBS.
0.05.M.+.NaCl.0.1.M,.pH.7;.inhibition.studies.
by.organic.phosphorous.compounds;.s:.whole.
blood
[2775]
Pepsin
Polyphenol.oxidase.(ads).+.
dialysis.membrane
Acetylene.carbon.black/
mo
CV,.A.(−0.05.V.vs..SSE)
det..via.released.tyrosine;.PBS.0.1.M.+.NaCl.
0.15.M,.pH.6.65
[779]
Plasmin
Adsorbed.on.CPE
G/Nj
UV-vis.photometry
Activity.study.with.H-D-val-leu-lys-
p
-
nitroanilide,.temperature,.pH.dependence
[2897]
Thermal.stability.
of.enzymes.in.
CPE
Rh
G/mo
Remarkable.stability.of.glucose.oxidase.in.CPE.
environment
[291,2707]
Thrombin
UV-vis.or.
electrochemical
det..via.release.of.amine.from.H-D-Phe-Pip-
Arg-4.MeO.β-naphthylamide,.2HCl
[2898]
Proteins and related compounds
Agglutinin.(wheat.
germ)
Chitin
acc.,.V
Selective.binding.to.
N
-acetyl.glucosamine.
residues.of.chitin
[2899]
Albumin
Amino.acids
Cu.microparticles
CNT/mo
acc..(−0.1.V.vs..SSE,.
10.min),.SWV
det..via.ox..of.amino.acids.in.protein;.0.05.M.
PBS,.pH.7.4
[2704]
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