Biomedical Engineering Reference
In-Depth Information
Fig. 8.
Transplantation of regenerated cardiomyocytes. Regenerated cardiomyocytes were trans-
planted into the adult mice heart by injection. The transplanted cardiomyocytes showed stable
long-term survival. After injection the regenerated cardiomyocytes adhered in small clusters to
surrounding cardiomyocytes, where they assumed the form of mature cardiomyocytes that had
the appearance of short strips of paper.
a
-
c
Cells in which the GFP fluorescence was observed;
d
-
h
cells transfected with the LacZ gene and stained.
i, j
Cells co-immunostained for con-
nexin43 formed apparent gap junctions with the surrounding cardiomyocytes. EGFP appears
green, nuclear staining with TOTO-3 is blue, and connexin43 labeling is red. (Reprinted and
modified from [14]).
