Biomedical Engineering Reference
In-Depth Information
Fig. 6.
ȕ
1-and
ȕ
2-adrenergic receptor expression and signal transduction in CMG cells.
a
Quan-
titative analysis of the expression of
ȕ
1-and
ȕ
2-adrenergic receptors. Their expression was nor-
malized relative to GAPDH. *P < 0.01 versus controls.
b
Cells were preincubated with
propranolol (10
-6
or 10
-5
mol/l) for 20 min and stimulated with isoproterenol (10
-7
mol/l) for 10
min.
c
Isoproterenol increased the spontaneous beating rate of CMG cells mainly via
ȕ
1 recep-
tors. CMG cells at 4 weeks after 5-azacytidine treatment were initially exposed to prazosin (10
-6
mol/l) for 30 min to block
ȕ
1-adrenergic receptors. Cells were then preincubated for 20 min with
vehicle (phosphate-buffered saline), propranolol, or CGP20712A, and then stimulated with iso-
proterenol. The beating rate was counted 3 min after stimulation. Phosphate-buffered saline was
added to the control.
