Chemistry Reference
In-Depth Information
Scheme 15.1 Schematic representation of the molecular imprinting process.
MIPs share the advantages of both biological and synthetic recognition platforms.
Like other synthetic polymer systems, MIPs possess excellent mechanical, chemical,
and thermal stability (Svenson and Nicholls 2001). However, they also possess the
ability to be tailored like biological systems with specificity for a molecule of interest.
For example, MIPs have been prepared with recognition properties for nucleotide
bases (Shea et al. 1993), pesticides (Yamazaki et al. 2001), amino acids (Lepisto
and Sellergren 1989), peptides (Hart and Shea 2002), pharmaceuticals (Vlatakis
et al. 1993), steroids (Cheong et al. 1997), sugars (Striegler 2003), and metal ions
(Uezu et al. 1997).
There are already several excellent reviews and monographs on MIPs (Wulff
1995; Sellergren 2001b; Yan and Ramstrom 2005; Alexander et al. 2006). Thus,
our focus will be on practical aspects of preparing and developing MIPs for
sensing applications (Takeuchi and Haginaka 1999; Haupt and Mosbach 2000;
Haupt 2001). This chapter will be divided into two parts. The first half (Sections
15.2-15.4) gives a general introduction to MIPs with a focus on preparation strat-
egies, recognition and material properties, and MIP formats. The second half
(Section 15.5) presents examples of MIP-based sensors. These include MIP
binding assays and mass sensitive, optical, and electrochemical sensors.
