Gels Mimicking Antibodies in Their Selective Recognition of Proteins and Its Potential Use for Protein Crystallization - Molecules: Nucleation, Aggregation and Crystallization - page 17

Fig. 3. Selective recognition of three proteins: hemoglobin (Hb), cytochrome c (C) and
lysozyme (L). Analysis in a cation exchanger of (a) the fraction collected from a blank
column (prepared in the absence of any protein) and (b) the fraction collected from the
column prepared in the presence of the sample. A comparison of the two chromatograms
indicates that hemoglobin, cytochrome c and lysozyme were adsorbed onto the column
prepared in the presence of these three proteins.
sodium phosphate, pH 6.2, and the eluate was analyzed at 220 nm by
cation-exchange chromatography (Fig. 3(b)). Evidently, all three proteins
had been adsorbed.
A similar experiment was performed on a blank column, i.e. a column
prepared in the absence of the three proteins (Fig. 3(a)). These figures show
that more than one protein can be adsorbed selectively on the same column.
In a pre-experiment on the same adsorbent we used glass wool to support
the bed. Because glass wool appeared to interact with hemoglobin, we
exchanged it for a piece of a nylon stocking in the runs described.