Crystallization of Proteins: Principles and Methods - Molecules: Nucleation, Aggregation and Crystallization - page 122

Fig. 6. A 24-well Linbro plate used in vapor diffusion from Molecular Dimensions Ltd.
(MD3-11ROWORLD) or Hampton Research Ltd. (HR3-110 or HR3-112).
protein solution and precipitating solution separated by a liquid interface.
As the two solutions diffuse into each other, the varying supersaturation
conditions lead to crystal growth at the interface between the two liquids
in the capillary. Free interface diffusion is one of the methods favored by
NASA for their microgravity crystallization research.
Granada crystallization box ® (GCB)
This is a simple device designed by Prof. Garcia-Ruiz and his co-workers
(Garcia-Ruiz et al ., 2002), used to crystallize protein and other biological
macromolecules by the counter-diffusion method (Fig. 7).
The bottom of the body of the box is filled with a layer of buffered
agarose gel at 0.5% w/v concentration. Capillaries of different inner
diameters ranging from 0.2 to 1.5 mm are used as the protein chamber.
Once the gel is set, the capillaries are filled with the protein and inserted
through the hole of the guide and punctuated to a given depth into the
gelled buffer layer. Finally, the crystallizing agent is poured on top of the
buffer gel.