Agriculture Reference
In-Depth Information
Table 9.2   (continued)
Plant
Androgenesis
(Explants)
Genetic transfor-
mation methods
Transformation freq.
(%): fertile plants/targets
References
AC
MC
-
MC
Microinjection
n.d
Gaillard et al. ( 1992 )
ADEs -
Electroporation
FTPs (5 %)
Sukhapinda et al.
( 1993 )
Rice
-
MDEs Particle
bombardment
FTPs (0-9.0 %)
Otani et al. ( 2005 )
ADEs -
Particle
bombardment
n.d
Baisakh et al. ( 2001 )
-
MDEs PGE
transformation
FTPs (0-47.0 %)
Chair et al. ( 1996 )
-
MC Agrobacterium FTPs (2.2 %)
Rashid et al. ( 1996 )
Triticale -
MDEs Particle
bombardment
FTPs (43-67.0 %)
Thmás ( 2003 )
-
MDEs Agrobacterium FTPs (3.3 %)
Zimny et al. ( 1995 )
Wheat ADEs -
Agrobacterium FTPs (2.38 %)
Chauhan and
Khurana ( 2010 )
-
MDEs Particle
bombardment
n.d
Gharanjik et al.
( 2008 )
ADEs -
Electroporation
n.d
Holiloglu et al.
( 2004 )
n.d Folling and Olesen
( 2002 )
ADEs - Agrobacterium n.d Pauk et al. ( 2003 )
ADEs - Agrobacterium FTPs (1.0-3.4 %) Massiah et al. ( 2001 )
ADEs - Agrobacterium n.d He et al. ( 1993 )
Rye - MDEs Agrobacterium n.d Kumlehn and Hensel
( 2009 )
AC Anther culture, MC Microspore culture, ADEs Anther-derived embryos, MDEs Protoplast iso-
lation for microspore-derived cell suspension cultures/Microspore-derived embryos, FTPs Fer-
tile transgenic plants, TF Transformation frequency: fertile plants/targets, n.d. = no data, √ = Yes,
- = No
-
MDEs Particle
bombardment
production of transgenic doubled haploid plant species in which regeneration from
somatic cells is difficult, especially in the recalcitrant cereals. Till now suitable ge-
netic transformation methods using different explants such as immature embryos,
mature embryos, scutellum-derived calli, anthers, isolated microspores, ovules, cell
suspensions, protoplast, etc., have been established for some cereal crops, especial-
ly for barley. Recent reports also focused on different cereal crops viz. rice, maize,
wheat, etc., which are mentioned under this review. By this time, many abiotic
stress inducible genes with various functions have been identified. Some research-
ers reported that transgenic plants that harbour these genes have shown increased
tolerance to cold, freezing, drought, salinity, heat, etc. Many reports have been pub-
lished on barley, but not enough on other cereal crops. It is time to work using stan-
dard protocols and need to develop specific abiotic stress tolerance cereal crops for
stressful soil and environments.
 
Search WWH ::




Custom Search