Agriculture Reference
In-Depth Information
Table 9.2
Application of androgenesis and genetic transformation methods to develop transgenic
doubled haploids in cereal crops
Plant
Androgenesis
(Explants)
Genetic transfor-
mation methods
Transformation freq.
(%): fertile plants/targets
References
AC
MC
Barley -
MC
Agrobacterium
n.d
Kumlehn and Hensel
(
2009
)
-
MDEs Particle
bombardment
FTPs (0-40.0 %)
Shim et al. (
2009
)
-
MDEs Particle
bombardment
n.d.
Obert et al. (
2008
)
-
MC
Agrobacterium
FTPs (0.3-2.2 %)
Kumlehn et al.
(
2006
)
-
MC
Agrobacterium
FTPs (17.64 %)
Coronado et al.
(
2005
)
-
MC
Agrobacterium
n.d
Hansel and Kumlehn
(
2004
)
-
MC
Agrobacterium
n.d
Kumlehn et al.
(
2004
)
-
MC
Biolistic
transformation
n.d
Chen et al. (
2003
)
-
MDEs Particle
bombardment
FTPs (43-67.0 %)
Thmás (
2003
)
MDEs Biolistic
transformation
FTPs (one plant per 10
-6
microspores)
Scholz et al. (
2001
)
-
MDEs Biolistic
transformation
FT (3.3 %)
Carlson et al. (
2001
)
-
MDEs
Agrobacterium
FTPs (0.3-2.9 %)
Wu et al. (
1998
)
-
MDEs Biolistic
transformation
FTPs (one plant per 10
-7
microspores)
Yao et al. (
1997
)
ADEs -
Particle
bombardment
n.d.
Koprek et al. (
1996
)
-
MC
Particle
bombardment
FTPs (one plant per 10
-7
microspores)
Jähne et al. (1994)
-
MDEs Particle
bombardment
TDHs (0.4 %)
Ritala et al. (
1994
)
-
MDEs
Agrobacterium
FTPs (0.3-7.9 %)
Wan and Lemaux
(
1994
)
-
MDEs Electroporation
FTPs (2.5 % plants per
10
-6
microspores)
Salmenkallio-Mart-
tila (
1994
)
-
MDEs
Agrobacterium
n.d
Kuhlmann et al.
(
1991
)
-
MDEs Microinjection
n.d
Bolik and Hu, (
1991
)
-
MDEs
Agrobacte-
rium
and
microprojectile
n.d
Creissen et al. (
1990
)
Maize
ADEs -
Biolistic
transformation
FTPs (45-61.0 %)
Aulinger et al. (
2003
)
-
MC
Electroporation
n.d
Fennell and Hanpt-
mann (
1992
)
