Agriculture Reference
In-Depth Information
Table 9.1
Application of Androgenetic Method for Improving Abiotic Stressed Cereal Crops
Plant
Name of stress
Androgene-
sis (Explants)
Results
(Embryos and
Regeneration)
References
AC MC
Barley Cold -
√ √
Davies and Morton (
1998
)
Cold √ - √ Jacquard et al. (
2003
);
Szarejko (
2003
)
Cold and or/starvation - √ √ Kasha et al. (
2001
)
Starvation
√
-
√
Castillo et al. (
2000
)
Durum Cold
√
-
√
Jauhar (
2003
)
Cold & osmotic stress √ Slama-Ayed et al. (
2010
)
Maize Cold
√
-
√
Barnabás (
2003
)
Colchicine
√
-
√
Obert and Barnabás (2004)
2-HNA and cold -
√ √
Zheng et al. (
2003
)
Oats Cold √ - √ Kiviharju and Pehu (
1998
)
Rice Cold
√
-
√
Zapata-Arias (
2003
)
Aluminum toxicity
√
-
√
Purwoko et al. (
2010
)
Rye Cold √ - √ Wenzel et al. (
1977
)
Osmotic stress -
√ √
Guo and Pulli (
2000
)
Triticale Cold
√
-
√
Tuvesson et al. (
2000
)
Cold - √ √ Pauk et al. (
2000
)
Wheat Cold
√
-
√
Tuvesson et al. (
2000
)
Drought
√
-
√
Chauhan and Khurana
(
2010
); Islam (
2010a
)
Heat and/or starvation -
√ √
Touraev et al. (
1996
)
Cold and/or starvation - √ √ Kasha et al. (
2003
)
Colchicine and heat
√
-
√
Barnabás (
2003
)
Colchicine -
√ √
Islam (
2010b
)
2-HNA - √ √ Zheng et al. (
2001
);
Liu et al. (
2001
)
Gametocide √ - √ Schmid and Keller (
1986
)
AC
Anther culture,
MC
Isolated microspore culture,
2-HNA
Hydroxynicotinic acid,
CGA
Game-
tocide, √ = Yes, - = No
3 Conclusion and Future Prospects
This review has been focused on combination of in vitro androgenesis and genetic
transformation for the production of fertile transgenic crops rapidly, using different
stress tolerance genes. Various stress pre-treatments, such as high temperature, cold,
heat shock, salt, drought, oxidative stress, reduced atmospheric pressure, osmotic
shock and starvation, during developmental of pollen grains are known to be essen-
tial for the induction of androgenesis in several crop plants, including cereals. Using
anther and microspore-derived embryos/callus/microspores/protoplast for genetic
transformation is an elegant system and could provide a practical alternative for the
